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Alteration of T Cell Phenotypes in HIV‐Neurotuberculosis Coinfection
Author(s) -
Rao Deepashri,
Venkataswamy Manjunatha M.,
Vasanthapuram Ravi,
Satishchandra P.,
Desai Anita
Publication year - 2020
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21746
Subject(s) - coinfection , cd8 , medicine , immunology , virology , flow cytometry , lentivirus , peripheral blood mononuclear cell , cytotoxic t cell , t cell , human immunodeficiency virus (hiv) , viral disease , immune system , biology , in vitro , biochemistry
Background Neurotuberculosis is one of the commonest HIV associated opportunistic infections of the central nervous system in India. HIV‐TB coinfection may lead to altered frequencies of T cells, thereby influencing the course and progression of the disease. Methods We examined the frequencies of T cell subsets in HIV infected individuals with neurotuberculosis (HIV+nTB+) as compared to individuals with HIV associated systemic TB (HIV+sTB+), asymptomatic HIV (HIV+TB‐), non‐HIV neuro TB (HIV‐nTB+), non‐HIV systemic TB (HIV‐sTB+), and healthy controls (HIV‐TB‐). Activation and senescence profiles of CD4 and CD8 T cells and memory subsets in peripheral blood mononuclear cells were studied by flow cytometry. Results The significant observations among the T cell subsets in HIV+nTB+ were: (1) Naïve T cells : decreased CD4 T cells compared to HIV‐sTB+ ( P = 0.005); decreased CD8 T cells compared to HIV‐nTB+ and HIV‐TB‐ ( P ≤ 0.007), (2) Memory T cells : expanded CD4 TEMRA cells compared to HIV‐nTB+, HIV‐sTB+, and HIV‐TB‐ ( P ≤ 0.003); expanded CD8 TEMRA cells compared to HIV‐nTB+ and HIV‐TB‐ ( P ≤ 0.005), (3) Activated T cells : higher CD4 T cells compared to HIV‐nTB+, HIV‐sTB+, and HIV‐TB‐ ( P ≤ 0.004); higher CD8 T cells compared to HIV + TB‐, HIV‐nTB+, HIV‐sTB+, and HIV‐TB‐ ( P ≤ 0.001), and (4) Senescent T cells : increased CD8 T cells compared to HIV‐nTB+ and HIV‐TB‐ groups ( P = 0.000). Conclusions Increased activation compared to HIV+TB‐, HIV‐nTB+, HIV‐sTB+, and HIV‐TB‐ groups and increased senescence compared to HIV‐nTB+ and HIV‐TB‐ groups were observed in CD8 T cells in HIV+nTB+, suggesting that the frequencies of these T cell subsets are altered to a greater extent in these individuals. © 2018 International Clinical Cytometry Society