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Evaluation of CD307a expression patterns during normal B‐cell maturation and in B‐cell malignancies by flow cytometry
Author(s) -
Auat Mariangeles,
Cardoso Chandra Chiappin,
SantosPirath Iris Mattos,
RudolfOliveira Renata Cristina Messores,
Matiollo Camila,
Lange Bárbara Gil,
da Silva Jessica Pires,
Dametto Gisele Cristina,
Pirolli Mayara Marin,
Colombo Maria Daniela Holthausen Perico,
SantosSilva Maria Claudia
Publication year - 2018
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21631
Subject(s) - flow cytometry , immunophenotyping , mantle cell lymphoma , chronic lymphocytic leukemia , b cell , lymphoma , pathology , cytometry , cell , hairy cell leukemia , biology , b cell lymphoma , leukemia , cancer research , medicine , immunology , antibody , genetics
Background Flow cytometric immunophenotyping is deemed a fundamental tool for the diagnosis of B‐cell neoplasms. Currently, the investigation of novel immunophenotypic markers has gained importance, as they can assist in the precise subclassification of B‐cell malignancies by flow cytometry. Therefore, the purpose of the present study was to evaluate the expression of CD307a during normal B‐cell maturation and in B‐cell malignancies as well as to investigate its potential role in the differential diagnosis of these entities. Methods CD307a expression was assessed by flow cytometry in normal precursor and mature B cells and in 115 samples collected from patients diagnosed with precursor and mature B‐cell neoplasms. CD307a expression was compared between neoplastic and normal B cells. Results B‐acute lymphoblastic leukemia cases exhibited minimal expression of CD307a, displaying a similar expression pattern to that of normal B‐cell precursors. Mantle cell lymphoma (MCL) cases showed the lowest levels of CD307a among mature B‐cell neoplasms. CD307a expression was statistically lower in MCL cases than in chronic B lymphocytic leukemia (CLL) and marginal zone lymphoma (MZL) cases. No statistical differences were observed between CD307a expression in neoplastic and normal plasma cells. Conclusion These results indicate that the assessment of CD307a expression by flow cytometry could be helpful to distinguish CLL from MCL, and the latter from MZL. Although these results are not entirely conclusive, they provide a basis for further studies in a larger cohort of patients. © 2018 International Clinical Cytometry Society