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Role of flow‐cytometric immunophenotyping in prediction of BCR/ABL1 gene rearrangement in adult B‐cell acute lymphoblastic leukemia
Author(s) -
Corrente Francesco,
Bellesi Silvia,
Metafuni Elisabetta,
Puggioni Pier Luigi,
Marietti Sara,
Ciminello Angela Maria,
Za Tommaso,
Sorà Federica,
Fianchi Luana,
Sica Simona,
De Stefano Valerio,
Chiusolo Patrizia
Publication year - 2018
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21605
Subject(s) - immunophenotyping , cd38 , breakpoint cluster region , cd33 , gene rearrangement , cd34 , fusion gene , flow cytometry , medicine , logistic regression , cd117 , oncology , leukemia , microbiology and biotechnology , pathology , cancer research , biology , gene , genetics , receptor , stem cell
We performed a retrospective analysis of 88 adult patients with B‐ALL diagnosed in our center by a flow‐cytometric assessment. Immunophenotypic expression of leukemic cells was explored by simultaneous evaluation of positivity, percentage of expressing cells and median fluorescence intensity (MFI). BCR/ABL1 fusion transcripts were assessed by RT‐PCR analysis and were identified in 36 patients (40.9%). CD10 and CD34 were positive in the totality of BCR/ABL1 ‐positive cases. Patients with gene rearrangement had a greater frequency of CD66c, CD13 and CD33 positivity compared with BCR/ABL1 ‐negative cases. Moreover, BCR/ABL1 ‐positive cases exhibited a greater median percentage and MFI values of CD13, CD33, CD66c, CD10, CD34 and CD25 expressions, but a lower median percentage and MFI values of CD38 and CD22 expressions than patients without gene rearrangement. Multivariate logistic regression analysis showed that CD10, CD38 and CD13 expressions were independent predictors for the presence of BCR/ABL1 rearrangement. Predictive probabilities of molecular occurrence based on these markers are proposed. © 2017 International Clinical Cytometry Society