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Simultaneous use of multiplex ligation‐dependent probe amplification assay and flow cytometric DNA ploidy analysis in patients with acute leukemia
Author(s) -
ReyesNúñez Virginia,
GaloHooker Evelyn,
PérezRomano Beatriz,
Duque Ricardo E.,
RuizArguelles Alejandro,
GarcésEisele Javier
Publication year - 2018
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21523
Subject(s) - multiplex ligation dependent probe amplification , aneuploidy , acute leukemia , multiplex , immunophenotyping , leukemia , flow cytometry , propidium iodide , medicine , microbiology and biotechnology , oncology , biology , immunology , bioinformatics , genetics , chromosome , gene , apoptosis , programmed cell death , exon
Background The aim of this work was to simultaneously use multiplex ligation‐dependent probe amplification (MLPA) assay and flow cytometric DNA ploidy analysis (FPA) to detect aneuploidy in patients with newly diagnosed acute leukemia. Methods MLPA assay and propidium iodide FPA were used to test samples from 53 consecutive patients with newly diagnosed acute leukemia referred to our laboratory for immunophenotyping. Results were compared by nonparametric statistics. Results The combined use of both methods significantly increased the rate of detection of aneuploidy as compared to that obtained by each method alone. The limitations of one method are somehow countervailed by the other and vice versa . Conclusions MPLA and FPA yield different yet complementary information concerning aneuploidy in acute leukemia. The simultaneous use of both methods might be recommended in the clinical setting. © 2017 International Clinical Cytometry Society