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Expression of CD43 in chronic lymphoproliferative leukemias
Author(s) -
Sorigue Marc,
Juncà Jordi,
Sarrate Edurne,
Grau Javier
Publication year - 2018
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21509
Subject(s) - cd43 , lymphoproliferative disorders , chronic lymphocytic leukemia , medicine , cd5 , immunophenotyping , flow cytometry , immunology , differential diagnosis , pathology , leukemia , lymphoma , cd20
Background CD43 has been used on histological samples for the differential diagnosis of lymphoproliferative disorders but there is scarce data on its use by flow cytometry (FC). We set out to characterize the expression of CD43 by FC in B‐cell lymphoproliferative disorders and to determine its possible role in the differential diagnosis of these malignancies. Methods We analyzed the expression of CD43 in clonal B‐cell lymphoproliferative disorders with exclusive peripheral blood and/or bone marrow involvement based on their Moreau chronic lymphocytic leukemia (CLL) score with particular emphasis on Moreau CLL score 3 (MS3) cases, which often present a diagnostic challenge. The cohort included 433 CLL (score 4–5), 34 MS3 and 166 lymphoproliferative disorders with lower scores. Results Generally, the higher the Moreau CLL score, the higher CD43‐positivity (425/443 [96%] for CLL, 23/34 [67%] for MS3 and 18/166 [11%] for cases with lower scores). MS3 cases constituted 5.4% of all cases and were more frequently CD5, CD200, CD43‐positive and had del(q13) than score 0–2 cases. Among MS3 cases, del(13q) cases were predominantly CD43‐positive (12/13). Conclusions The frequency of CD43‐positivity increases sharply with the Moreau score. MS3 cases seem to include both CLL and non‐CLL lymphoproliferative disorders and CD43 could aid in the differential diagnosis between the two. However, studies analyzing the correlation between CD43 expression and the underlying biologic changes of these cases are warranted. © 2017 International Clinical Cytometry Society