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Elevated IL‐17A and IL‐22 regulate expression of inducible CD38 and Zap‐70 in chronic lymphocytic leukemia
Author(s) -
Kouzegaran Samaneh,
Siroosbakht Soheila,
Farsad Bahram Fariborz,
Rezakhaniha Bijan,
Dormanesh Banafshe,
Behnod Vahid,
Tanha Amir Saber
Publication year - 2018
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21487
Subject(s) - chronic lymphocytic leukemia , cd38 , medicine , interleukin 17 , gastroenterology , pathogenesis , interleukin , leukemia , immunology , inflammation , cytokine , biology , cd34 , stem cell , genetics
Background In this study, we investigated the role and expression of interleukin (IL)−17A and IL‐22 in chronic lymphocytic leukemia. Methods We evaluated the expression of markers above on CLL by ELISA, qRT‐PCR, flow cytometric analysis and nonparametric Kruskal–Wallis test. Results Quantitative RT‐PCR revealed that the mRNA levels of IL‐17A and IL‐22 in PBMCs of CLL patients were upregulated compared with those from healthy subjects (mean ± SD: 1.96 ± 0.232 vs.0.72 ± 0.15, P < 0.001 and mean ± SD: 2.45 ± 0.534 vs.0.81 ± 0.26, P < 0.001, respectivily). In addition, findings showed that the IL‐17A and IL‐22 plasma level was significantly elevated than that from healthy control group ( P < 0.001). The median IL‐17A and IL‐22 in CLL patients and healthy control group were 48.28 ± 17.2 pg mL −1 ; 20.01 ± 11.16 pg mL −1 and 58.68 ± 23.4 pg mL −1 ;16.47 ± 10.31 P < 0.001, respectively. The levels of IL‐17A and IL‐22 was not significantly associated with the different stages of disease (Rai stages; Kruskal–Wallis test P > 0.05).No significant relationship was found between expression of CD38 and higher median serum levels of IL‐17A in patients, but patients with negative expression of ZAP‐70 showed a significant association with higher median serum levels of IL‐17A compared with healthy subjects. (57.84 pg mL −1 vs. 31.67 pg mL −1 ; P = 0.016). Conclusion IL‐22 is elevated and associated with CD38 and Zap‐70 expression in patients with CLL. No significant correlation was found between expression of CD38 and increased levels of IL‐17A, negative expression of ZAP‐70 showed a significant association with increased levels of IL‐17A. © 2016 International Clinical Cytometry Society