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Consumption of a high‐fat meal was associated with an increase in monocyte adhesion molecules, scavenger receptors, and Propensity to Form Foam Cells
Author(s) -
Henning A. L.,
Venable A. S.,
Vingren J. L.,
Hill D. W.,
McFarlin B. K.
Publication year - 2018
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21478
Subject(s) - cd36 , foam cell , scavenger receptor , cd14 , monocyte , chemistry , cell adhesion molecule , postprandial , medicine , endocrinology , meal , cell adhesion , lipoprotein , adhesion , receptor , biology , cholesterol , immunology , food science , biochemistry , insulin , organic chemistry
Background Macrophage‐derived foam cells are the predominant component of arterial plaques in the early stages of atherosclerosis. One factor that poses a major risk for plaque development is high levels of plasma low‐density lipoprotein (LDL) as a result of a high‐fat meal. In order to better understand how an individuals' diet affects arterial plaque deposition via the process of foam cell formation, we measured the acute circulating monocyte activity response after consuming a high‐fat meal (85% of daily fat allowance). Materials and Methods Venous blood samples from 17 participants were acquired on a FlowSight. Samples were analyzed to identify nonclassical (CD14+/16+) and classical (CD14+/16−) monocytes. We measured monocyte concentration, adhesion molecule expression, CD36 expression, and oxidized LDL (oxLDL) endocytosis for preprandial 1, 3, and 5 h postprandial. Results Consuming a high‐fat meal caused increases in oxLDL uptake, adhesion molecule expression, and CD36 expression in both classical and nonclassical monocytes, with the nonclassical monocytes responding with larger increases than the classical monocytes. Conclusion These results suggest that consumption of a high‐fat meal increased the potential of monocytes to become foam cells, and implicates nonclassical monocytes as having greater potential than classical monocytes to become foam cells. © 2016 International Clinical Cytometry Society