Premium
Determination of blood cell subtype concentrations from frozen whole blood samples using TruCount beads
Author(s) -
Langenskiöld Cecilia,
Mellgren Karin,
Abrahamsson Jonas,
Bemark Mats
Publication year - 2018
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21390
Subject(s) - flow cytometry , whole blood , chromatography , blood collection , cytometry , chemistry , andrology , biology , medicine , microbiology and biotechnology , immunology , emergency medicine
Background In many studies it would be advantageous if blood samples could be collected and analyzed using flow cytometry at a later stage. Ideally, sample collection should involve little hands‐on time, allow for long‐term storage, and minimally influence the samples. Methods Here we establish a flow cytometry antibody panel that can be used to determine granulocytes, monocytes, and lymphocyte subset concentrations in fresh and frozen whole blood using TruCount technology. Results The panel can be used on fresh whole‐blood samples as well as whole‐blood samples that have been frozen after mixing with 10% DMSO. Concentrations in frozen and fresh sample is highly correlated both when frozen within 4 h and the day after collection ( r ≥ 0.98), and the estimated concentration in frozen samples was between 91 and 94% of that in fresh samples for all cell types. Conclusion Using this method whole‐blood samples can be frozen using a simple preparation method, and stored long‐term before accurate determination of cell concentration. This allows for standardized analysis of the samples at a reference laboratory in multi‐center studies. © 2016 International Clinical Cytometry Society