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Impact of T cell activation, HIV replication and hepatitis C virus infection on neutrophil CD64 expression
Author(s) -
Morquin D.,
Tuaillon E.,
Makinson A.,
Bendriss S.,
Le Moing V.,
Reynes J.
Publication year - 2017
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21385
Subject(s) - cd64 , immunology , cd8 , immune system , medicine , flow cytometry , virology , biology
Background Overexpression of the Fc receptor CD64 on neutrophils is associated with innate immune response and bacterial infections. During HIV infection a large set of immune disorders including T‐lymphocyte over‐activation, microbial translocation, impairment of neutrophil functions, and immunodeficiency may interplay with neutrophil CD64 expression. Methods Associations of neutrophil CD64 expression with CD8 + T cell activation, CD4 + T cells number, HIV, and HCV replications were investigated in HIV infected patients using a standardized method. Results Higher neutrophil CD64 expression was observed in HIV infected subjects compared to healthy controls (0.91 vs. 0.75, P < 0.001). Among 115 HIV infected patients, nine (8.8%) had a CD64 expression over the clinical threshold as calculated against bead standard (i.e., 1.5). HIV viremic patients were more likely to have an index above 1.5 (OR: 6.68, P values: 0.01). A trend for correlation between CD64 expression and CD8 T cell activation was observed ( P values: 0.08). Blood CD4 + T lymphocyte depletion and HCV replication did not affect neutrophil CD64 expression. Conclusions HIV infection and HIV replication are associated with up‐regulation of neutrophil CD64. CD64 overexpression above the clinical threshold was observed in a minor proportion of HIV infected individuals treated by antiretroviral therapy and may be a marker of neutrophil activation related to non‐AIDS‐linked comorbidities. © 2016 International Clinical Cytometry Society