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CD 180 overexpression in follicular lymphoma is restricted to the lymph node compartment
Author(s) -
Mestrallet Fanélie,
Sujobert Pierre,
Sarkozy Clémentine,
TraverseGlehen Alexandra,
CalletBauchu Evelyne,
Magaud JeanPierre,
Salles Gilles,
Baseggio Lucile
Publication year - 2016
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21331
Subject(s) - chronic lymphocytic leukemia , follicular lymphoma , mantle cell lymphoma , lymphoma , marginal zone b cell lymphoma , marginal zone , flow cytometry , lymph node , pathogenesis , lymph , lymphoproliferative disorders , follicular phase , medicine , pathology , biology , b cell , immunology , leukemia , antibody
Background Altered Toll‐like receptor (TLR) expression levels and/or mutations in its signaling pathway (such as MyD88 mutation) contribute to the pathogenesis of lymphoproliferative disorders (LPD). CD180 is an orphan member of the TLR family that modulates the signaling of several TLRs, but only limited studies have evaluated its expression by flow cytometry (FCM) in LPD. Methods Using a multiparameter FCM approach, we have assessed CD180 mean fluorescence intensity (MFI) in lymph nodes (LNs) and peripheral blood (PB) samples obtained from patients with follicular lymphoma (FL; LN/PB, n = 44/ n = 15), chronic lymphocytic leukemia (CLL, n = 26/ n = 21), mantle cell lymphoma (MCL, n = 13/ n = 17), and marginal zone lymphoma (MZL, n = 16/ n = 12). Specimens from non‐tumoral PB and LN ( n = 8/ n = 12) were used as controls. Results In the LN specimens, FL and control B‐cells showed similar CD180 expression (MFI = 1,049 vs. 1,381, P > 0.05; Mann–Whitney U ‐test). This level was markedly lower in the other LPDs, MCL (MFI = 396, P < 0.05), or CLL (MFI = 502 P < 0.05), and similar to MZL (MFI = 858, P > 0.05). However, the CD180 expression of FL B‐cells assessed in PB was dim and/or negative, in the same range as MCL and CLL (FL MFI = 453, MCL MFI = 305, CLL MFI = 420, P > 0.05) but lower than in MZL (MFI = 895, P < 0.05). Therefore, these results suggest a modulation of CD180 expression by neoplastic FL B‐cells based on the anatomical compartment. Conclusion These FCM data confirm the usefulness of CD180 in the accurate diagnosis of LPDs and emphasize the need to interpret this marker according to the origin of the sample. © 2015 Clinical Cytometry Society