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Optimization of EGFR high positive cell isolation procedure by design of experiments methodology
Author(s) -
Levi Ofer,
Tal Baruch,
Hileli Sagi,
Shapira Assaf,
Benhar Itai,
Grabov Pavel,
Eliaz Noam
Publication year - 2015
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21246
Subject(s) - circulating tumor cell , isolation (microbiology) , blood circulation , recovery rate , immunomagnetic separation , metastasis , medicine , computer science , biology , chemistry , chromatography , bioinformatics , cancer , traditional medicine
Background Circulating tumor cells (CTCs) in blood circulation may play a role in monitoring and even in early detection of metastasis patients. Due to the limited presence of CTCs in blood circulation, viable CTCs isolation technology must supply a very high recovery rate. Methods Here, we implement design of experiments (DOE) methodology in order to optimize the Bio‐Ferrography (BF) immunomagnetic isolation (IMI) procedure for the EGFR high positive CTCs application. All consequent DOE phases such as screening design, optimization experiments and validation experiments were used. Results A significant recovery rate of more than 95% was achieved while isolating 100 EGFR high positive CTCs from 1 mL human whole blood. Conclusions The recovery achievement in this research positions BF technology as one of the most efficient IMI technologies, which is ready to be challenged with patients' blood samples. © 2015 International Clinical Cytometry Society