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Introduction to the diagnosis and classification of monocytic‐lineage leukemias by flow cytometry
Author(s) -
Matarraz Sergio,
Almeida Julia,
FloresMontero Juan,
Lécrevisse Quentin,
Guerri Valentina,
López Antonio,
Bárrena Susana,
Van Der Velden Vincent H. J.,
Te Marvelde Jeroen G.,
Van Dongen Jacques J. M.,
Orfao Alberto
Publication year - 2017
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21219
Subject(s) - flow cytometry , bone marrow , haematopoiesis , immunophenotyping , pathology , cd34 , differential diagnosis , myeloid , lineage (genetic) , identification (biology) , biology , immunology , medicine , cancer research , stem cell , microbiology and biotechnology , gene , genetics , botany
Despite diagnostic criteria are currently available for the distinct subtypes of monocytic‐lineage neoplasias, a number of partially overlapping features still remain evident, which may hamper their differential diagnosis. An accurate identification and characterization of monocytic cells is of major relevance for the diagnosis and classification of these neoplasias. In this regard, as compared to other conventional techniques, flow cytometry has shown the highest sensitivity for detection of early monocytic commitment of (normal and neoplastic) bone marrow CD34 + hematopoietic precursors as well as of monocytic aberrations and maturation blockades, which are frequently associated with clonal myeloid disorders. In the present paper we provide basic principles and criteria for multiparameter flow cytometry identification and characterization of bone marrow monocytic cells that contribute to an improved diagnostic and classification of monocytic lineage‐associated acute leukemias in clinical settings, particularly when using the EuroFlow antibody panels. © 2015 International Clinical Cytometry Society