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Adult T‐cell leukemia/lymphoma with an unusual CD1a positive phenotype
Author(s) -
Juncà Jordi,
Botín Teresa,
Vila Jordi,
Navarro JosepTomàs,
Millá Fuensanta
Publication year - 2014
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21130
Subject(s) - cd5 , lymphoma , bone marrow , cd8 , pathology , leukemia , cd34 , t cell , biology , phenotype , t cell leukemia , lymph node , immunophenotyping , medicine , immunology , cancer research , flow cytometry , antigen , immune system , stem cell , gene , biochemistry , genetics
Background Acute T‐cell leukemia lymphoma (ATLL) tumor cells generally express CD2/CD3/CD5, but lack CD7. These T cells are usually CD4+CD8− and strongly express CD25, although some variability in this basic pattern may be found. Here we report a case with a very unusual CD1a positive phenotype. Methods Samples from peripheral blood, bone marrow aspirate, lymph node, and cerebrospinal fluid obtained from a 45‐year‐old male patient with a T‐cell lymphoproliferative disorder were immunophenotyped by multiparametric flow cytometry. Analysis of HTLV‐I genome integration in tumoral cells was performed by PCR. Results Neoplastic T cells were cCD3, CD2/CD5/CD30/CD25, and CD1a positive, but CD3/CD7/CD4/CD8/CD34/CD10/TdT negative. Serology and integration of HTLV‐I were positive. Conclusion To the best of our knowledge, CD1a expression has not been previously described in this entity. Its detection raised the differential diagnosis with acute T lymphoblastic leukemia. The rest of the phenotypic markers, the morphology of the neoplastic cells, and the demonstration of HTLV‐I genome integration provided the final diagnosis. © 2013 International Clinical Cytometry Society