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CD200 has an important role in the differential diagnosis of mature B‐cell neoplasms by multiparameter flow cytometry
Author(s) -
Sandes Alex F.,
de Lourdes Chauffaille Maria,
Oliveira Cláudia Regina M.C.,
Maekawa Yumi,
Tamashiro Nair,
Takao Tânia T.,
Ritter Estela C.,
Rizzatti Edgar G.
Publication year - 2014
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21128
Subject(s) - chronic lymphocytic leukemia , mantle cell lymphoma , flow cytometry , cd5 , hairy cell leukemia , cd11c , differential diagnosis , immunophenotyping , cancer research , lymphoma , antibody , b cell , leukemia , pathology , biology , medicine , immunology , phenotype , genetics , gene
Background Multiparameter flow cytometry is a useful tool for the diagnostic evaluation of mature B‐cell neoplasms (MBN). Recently, it has been shown that CD200 may improve the distinction between chronic lymphocytic leukemia (CLL; CD200+) and mantle cell lymphoma (MCL; CD200−), but the role of CD200 expression in atypical CLL and other MBN remains to be established. Methods To address this issue, we investigated the expression of CD200 in 159 consecutive cases of MBN. Results CD200 was strongly expressed in CLL and was revealed to be an excellent marker to distinguish CLL from MCL, even in cases of atypical CLL. However, lack of CD200 was not an exclusive finding of MCL, being also observed in other MBNs. Furthermore, CD200 was highly expressed in hairy cell leukemia, being useful in the differential diagnosis of lymphomas with villous lymphocytes. Herein, we propose an algorithm to classify CD5+ MBNs based on the expression of CD200, CD11c, heavy chain immunoglobulins, and Matutes score. Conclusions These results expand the understanding of the CD200 expression in MBNs, giving further support for the inclusion of this marker in the routine investigation by flow cytometry. © 2013 International Clinical Cytometry Society