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Value of HIV patients with regular follow‐up as In‐house Internal Controls of Flow Cytometry Measurement of Lymphocyte Subsets
Author(s) -
Carvalho Bittencourt Marcelo,
Kohler Chantal,
Henard Sandrine,
Rabaud Christian,
Béné Marie C.,
Faure Gilbert C.
Publication year - 2013
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21112
Subject(s) - flow cytometry , repeatability , cd8 , cytometry , reproducibility , human immunodeficiency virus (hiv) , medicine , cd3 , immunology , coefficient of variation , mathematics , statistics , immune system
Background Quality assessment in flow cytometry cannot obey the same rules as those applicable to the measurement of chemical analytes. However, regular follow‐up of known patients may provide a robust in‐house control of cell subsets evaluation. Methods Sequential blood samples assessed for 32 HIV patients over several years and showing good stability were retrospectively assessed to establish coefficient of variations of the percentages of CD3+, CD4+, CD8+ cells, and CD4+ absolute counts (ACs). Results Mean relative standard variations for the whole cohort were of 0.04, 0.14, 0.08, and 0.18 for CD3%, CD4%, CD8%, and CD4 ACs, respectively. Discussion In‐house follow‐up of regularly checked compliant patients is a good alternative to traditional and costly repeatability and reproducibility studies for the validation of routine flow cytometry. © 2013 International Clinical Cytometry Society