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Altered T cell differentiation associated with loss of CD27 and CD28 in HIV infected Indian individuals
Author(s) -
Mojumdar Kamalika,
Vajpayee Madhu,
Chauhan Neeraj Kumar,
Singh Alpana,
Singh Ravinder,
Kurapati Sravya
Publication year - 2012
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.20610
Subject(s) - cd28 , immunology , immunosenescence , cd8 , biology , t cell , viremia , naive t cell , population , cellular differentiation , immune system , cytotoxic t cell , human immunodeficiency virus (hiv) , medicine , in vitro , genetics , t cell receptor , environmental health , gene
Background: HIV‐1 infection is associated with depletion of naïve T cell subsets and skewed T cell differentiation and maturation, leading to accumulation of T cells at intermediate and end stages of differentiation. CD27 and CD28 expression have been utilized in assessing these population subsets. Methods: We characterized T cell subsets based on expression of CD45RA, CCR7, CD27, and CD28 and compared these subsets in HIV‐1 infected Indian subjects and uninfected controls. Results: HIV‐1 infection was associated with an increase in effector and memory T cell subsets and a concomitant decrease in naïve T cells. HIV‐1 infected subjects showed accumulation of intermediate CD8 T cell (CD27+CD28−) differentiation subsets, whereas CD4 T cells progressed to late stage differentiation (CD27−CD28−). These subsets were negatively associated with CD4 T cell counts and positively associated with plasma viremia. CD57, an immunosenescence marker, was also increased on T cell subsets from HIV‐1 infected individuals. Antiretroviral therapy resulted in partial restoration of differentiation status. Conclusion: Persistent HIV‐1 replication and chronic immune activation, along with altered cytokine secretion profile, lead to impaired T cell differentiation and maturation. Detailed understanding of factors associated with differentiation defects in HIV‐1 infected Indian individuals will strongly assist in Indian HIV‐1 vaccine efforts and add to our knowledge of HIV‐1 subtype C pathogenesis. © 2011 International Clinical Cytometry Society