Premium
Increased polyclonal CD5 + B1a lymphocytes in a haploidentical stem cell transplant recipient
Author(s) -
Hillard Robert A.,
Lekakis Lazaros J.,
Pulliam Joseph F.
Publication year - 2011
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.20572
Subject(s) - cd5 , polyclonal antibodies , stem cell , immunology , population , lymphocyte , medicine , stromal cell , transplantation , biology , pathology , flow cytometry , antibody , microbiology and biotechnology , environmental health
Abstract Background: Atypical lymphocyte populations may be seen in the peritransplant setting. In this case report, we describe an unusually high number of CD5 + B‐cells (B1a cells) following transplant. Methods: B1a cells identified during routine follow‐up by immunophenotypic analysis in a middle‐aged man who had a haploidentical stem cell transplant for acute myeloid leukemia were compared with a reference set of post‐transplant samples. Results: Increased but polyclonal B1a cells were identified with 100% donor chimerism. Conclusions: Our case demonstrates that a high absolute number of B1a cells may be seen post‐transplant and should not be confused with an atypical CD5 + lymphoproliferative disorder. Furthermore, the population of polyclonal CD5 + B lymphocytes from the patient's donor is prominent 7 months post‐transplant. This suggests that the maintenance of CD5 + B1 cells prior to conversion to adult‐type CD5 − B2 cells is not hindered by the recipient adult stromal environment. © 2010 International Clinical Cytometry Society