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Absolute CD4 + cell count using a plastic microchip and a microscopic cell counter
Author(s) -
Bae Sook Young,
Park Hae Chul,
Oh Jun Seo,
Yoon SooYoung,
Park Dae Won,
Choi In Keun,
Kim Hae Joong,
Oh Jong Hyun,
Hur Dae Sung,
Chung Chanil,
Chang Jun Keun,
Robinson J. Paul,
Lim Chae Seung
Publication year - 2009
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.20470
Subject(s) - absolute (philosophy) , physics , materials science , epistemology , philosophy
We have designed and evaluated the performance of a simple, rapid, and affordable method for counting CD4 + T‐cells with the use of plastic microchips. This new system is an adaptation of a “no‐lyse, no‐wash,” volumetric single platform assay, and absolute CD4 + counts are determined with the use of a microscopic scanning cell counter. To assess the CD4 + count test precision and linearity of the system, measured CD4 + counts were compared with two other reference assays (single and dual platform flow cytometry) with the use of 123 clinical samples including samples obtained from 35 HIV‐infected patients, and artificially diluted samples. A correlation between the results from the use of the new method and from the use of the two other reference assays was r = 0.98 for the clinical samples. A dilution test of the new method demonstrated a linearity of r ≥ 0.99, with coefficients of variation ≤7.6% for all concentration levels. Our findings suggest that the new CD4 + counting device can be potentially be applied for other diagnostic procedures that measure quantities of characteristic antigens or other materials on cells. © 2009 Clinical Cytometry Society