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Monocytes and T lymphocytes contribute to a predominance of interleukin 6 and interleukin 10 in systemic lupus erythematosus
Author(s) -
MellorPita Susana,
Citores Maria J.,
Castejon Raquel,
YebraBango Miguel,
TutorUreta Pablo,
Rosado Silvia,
Andreu Jose L.,
Vargas Juan A.
Publication year - 2009
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.20468
Subject(s) - interleukin 10 , ionomycin , cytokine , cd8 , immunology , interferon gamma , medicine , tumor necrosis factor alpha , interleukin 4 , monocyte , endocrinology , immune system , stimulation
Objective To investigate the contribution of T lymphocytes and monocytes to cytokine production in systemic lupus erythematosus (SLE). Methods Forty‐five SLE patients and 19 healthy volunteers were included. Serum levels of tumor necrosis factor alpha (TNFα), interferon gamma (IFNγ), interleukin (IL)‐6, and IL10 were quantified by ELISA. The cytokine production capacities of peripheral blood mononuclear cells were assessed by culturing in vitro with PMA+Ionomycin or LPS. The intracellular cytokine expression was measured by flow cytometry in T lymphocytes and monocytes, respectively. The influence of the disease activity (measured as the SLE‐disease activity index; SLEDAI) and the treatment the patients were receiving was evaluated. Results Serum IL10, IL6, and TNFα levels were increased in patients ( P ≤ 0.01), and a higher spontaneous (without stimuli) intracellular expression of IL10 in CD4+ and CD8+ T lymphocytes ( P < 0.05) and of IL6 in monocytes ( P = 0.01) were found. After stimulation, patients presented a higher percentage of CD4+ and CD8+ T lymphocytes producing IL4 and IL10 ( P ≤ 0.01), and of monocytes producing IL6 ( P = 0.04) and IL10 ( P = 0.008). The SLEDAI score was positively correlated with the percentage of CD4+IL10+ and CD8+IL10+ T lymphocytes ( P < 0.01), and inversely correlated with CD8+TNFα+ ( P = 0.02), CD4+IFNγ+ ( P = 0.04) and CD8+ IFNγ+ ( P = 0.002) T lymphocytes. Patients receiving high dose prednisone produced higher IL10, but they also were the patients with a more active disease. Conclusion Monocytes and T lymphocytes (CD4+ and CD8+) contribute to an overproduction of IL6 and IL10 in SLE; this correlates with the disease activity but is independent of the treatment the patients are receiving. © 2009 Clinical Cytometry Society