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Affordable pediatric CD4 counting by flow cytometry in Malawi
Author(s) -
MacLennan Calman A.,
Dzumani Felix,
Namarika Alinane,
Moons Peter,
Senga Edward,
Molyneux Malcolm E.,
Drayson Mark T.,
Bunn James E. G.
Publication year - 2008
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.20411
Subject(s) - flow cytometry , limits of agreement , medicine , coefficient of variation , human immunodeficiency virus (hiv) , lymphocyte , immunology , nuclear medicine , chemistry , chromatography
Background Rapid expansion of antiretroviral therapy in Malawi has occurred in the relative absence of suitable pediatric CD4 counting facilities. We have recently validated in adults a simplified affordable flow cytometric CD4 counting method, the Blantyre count. There is a need for this technology to transfer to government laboratories run by local staff, and to be validated in children, where %CD4/lymphocyte values are required. Methods We assessed agreement of %CD4/lymphocyte values determined by the Blantyre count and Panleucogate methods on an EPICS XL‐MCL flow cytometer on 113 venous blood samples from HIV‐seropositive children in Blantyre, Malawi. All assays were performed by two Malawian laboratory technicians. Results Overall bias between the two methods was −0.13% (95% CI −0.37 to 0.11) and limits of agreement were −2.69 to 2.43% (95% CI −3.11 to −2.27 and 2.01 to 2.85). Limits of agreement were within −3.00 and 3.00 for each laboratory technician. Coefficient of variation for the Blantyre count assay was 2.0% and samples showed good stability over 5 days. Conclusions The Blantyre count method can accurately determine %CD4/lymphocyte values in blood of HIV‐seropositive children on an EPIC XL‐MCL flow cytometer at a reagent cost of US $0.21 per test or less. The assay can be competently carried out by local laboratory technicians. © 2008 Clinical Cytometry Society