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Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells
Author(s) -
Gaipa Giuseppe,
Basso Giuseppe,
Aliprandi Sara,
Migliavacca Maddalena,
Vallinoto Cristina,
Maglia Oscar,
Faini Andrea,
Veltroni Marinella,
Husak Dzvenyslava,
Schumich Angela,
Ratei Richard,
Biondi Andrea,
Dworzak Michael N.
Publication year - 2008
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.20408
Subject(s) - prednisone , cd34 , flow cytometry , immunophenotyping , bone marrow , antigen , medicine , leukemia , acute leukemia , cancer research , immunology , microbiology and biotechnology , pathology , biology , stem cell
Background: Immunophenotypic modulation is induced by steroids in pediatric B‐cell precursor acute lymphoblastic leukemia (BCP‐ALL) patients during remission induction therapy. Methods: We cultured BCP‐ALL blasts from diagnostic bone marrow (BM) samples ( n = 20) in the presence of prednisone on stroma layer obtained from BM‐derived mesenchymal cells to maintain viability. Antigen expression was assessed by multiparametric flow cytometry. Results: Leukemia samples that sustained the treatment in vitro with prednisone, showed significative reduction of CD10 and CD34 expression compared with control, and it was comparable with that observed in residual leukemic cells of the same patients in BM at day 15 of treatment. Modulated cells were viable as determined by Annexin V staining and preserved light scattering properties. Of note, the extent of antigen modulation in vitro correlated with response to prednisone in vivo. Conclusions: The prednisone‐induced immunophenotypic modulation can be reproduced in vitro and this phenomenon may reflect sensitivity to chemotherapy. © 2008 Clinical Cytometry Society