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Clone‐specific anti‐CD45 blocking factor in patient plasma
Author(s) -
Wellhausen Samuel R.,
Slone Stephen P.,
Miller James J.
Publication year - 2007
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.20168
Subject(s) - blocking (statistics) , clone (java method) , staining , cd3 , antibody , immunofluorescence , microbiology and biotechnology , chemistry , cd8 , antigen , immunology , biology , pathology , medicine , biochemistry , dna , computer science , computer network
We observed inhibition of anti‐CD45, but not anti‐CD3, ‐CD4, or ‐CD8 staining in the whole blood of an individual clinical specimen. Subsequent experiments demonstrated the presence of a plasma factor, which likewise blocked CD45 staining of third party lymphocytes. The blocking activity was anti‐CD45 clone (2D1) specific. Experiments utilizing immunoabsorption, immunofluorescence, and commercial blocking reagents failed to provide evidence for identifying blocking factor as heterophilic antibody. Biochemical identification of blocking factor was not accomplished. © 2007 Clinical Cytometry Society.