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Expression of inhibitory receptor ILT3 on neoplastic B cells is associated with lymphoid tissue involvement in chronic lymphocytic leukemia
Author(s) -
Colovai Adriana I.,
Tsao Lawrence,
Wang Su,
Lin Hana,
Wang Chuan,
Seki Tetsunori,
Fisher Julie G.,
Menes Manuel,
Bhagat Govind,
Alobeid Bachir,
SuciuFoca Nicole
Publication year - 2007
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.20164
Subject(s) - chronic lymphocytic leukemia , cd5 , flow cytometry , leukemia , immune system , antigen , antigen presenting cell , biology , cancer research , immunology , t cell
T cell responses against leukemia‐associated antigens have been reported in chronic lymphocytic leukemia (CLL). However, the relentless accumulation of CLL B cells in some patients indicates that anti‐tumor immune responses are inefficient. Inhibitory receptors from the Ig‐like transcript (ILT) family, such as ILT3 and ILT4, are crucial to the tolerogenic activity of antigen presenting cells. In this study, we examined the expression of ILT3 on CD5+ B cells obtained from 47 patients with CLL. Using flow cytometry and RT‐PCR, we found that B CLL cells from 23 of 47 patients expressed ILT3 protein and mature ILT3 mRNA. ILT3 protein and mRNA were not found in normal B cells obtained from donors without CLL. Expression of ILT4 in normal and B CLL cells showed a pattern similar to ILT3. The frequency of ILT3 positive CLL B cells was higher in patients with lymphoid tissue involvement, suggesting that ILT3 may have prognostic value in CLL. Our findings indicate that expression of ILT3 and ILT4 on CLL B cells represents a phenotypic abnormality that may play a role in tolerization of tumor‐specific T cells. © 2007 Clinical Cytometry Society