Premium
Sterile and disposable fluidic subsystem suitable for clinical high speed fluorescence‐activated cell sorting
Author(s) -
Jayasinghe Sachintha M.,
Wunderlich Joshua,
McKee Angel,
Newkirk Heather,
Pope Steve,
Zhang Jiwang,
StaehlingHampton Karen,
Li Linheng,
Haug Jeffrey S.
Publication year - 2006
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.20111
Subject(s) - aseptic processing , fluidics , cell sorting , modular design , microfluidics , isolation (microbiology) , sorting , computer science , flow cytometry , biomedical engineering , biology , microbiology and biotechnology , nanotechnology , engineering , materials science , immunology , mechanical engineering , operating system , programming language , aerospace engineering
Background: Applications of fluorescence‐activated cell sorting (FACS) are ideally performed under aseptic conditions so that isolated cells can be successfully cultured, transplanted, or processed for the isolation of protein and nucleic acids. However, modern “off‐the shelf” flow cytometers are suboptimally designed for these purposes because nonsterile instrument hardware components directly contact sample‐harboring fluids, compromising their sterility. Methods: We have described the design and modular modification of a cytometer with a sterile and disposable FACS fluid handling system that meets requirements of high‐speed FACS and good manufacturing practice. This system was tested for functionality and its ability to maintain a clean and sterile fluid environment. Results: Our data have shown that this new fluidic subsystem completely replicated the intended function of the manufacturer's standard fluid handling system, and isolates the fluid from contaminants such as bacteria and fungus, endotoxins, mycoplasma, and helicobacter. Conclusions: FACS has emerged as a powerful tool used to study and manipulate stem cells. However, if stem cell discoveries are to be fully utilized in clinical transplant medicine, aseptic instrument configurations must be developed. For this purpose, we have designed a disposable sterile fluid handling system. © 2006 International Society for Analytical Cytology