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Detection of circulating endothelial cells and endothelial progenitor cells by flow cytometry
Author(s) -
Khan Sameena S.,
Solomon Michael A.,
McCoy J. Philip
Publication year - 2005
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.20040
Subject(s) - progenitor cell , flow cytometry , endothelial stem cell , biology , microbiology and biotechnology , immunology , vasculogenesis , circulating tumor cell , stem cell , in vitro , cancer , metastasis , biochemistry , genetics
Abstract The finding of angiogenic and vasculogenic cells in the peripheral circulation may have profound effects on the course of a variety of diseases ranging from cancer to cardiovascular disease. These cells are ascribed to be endothelial in nature and are generally referred to as circulating endothelial cells if mature or as endothelial progenitor cells if immature. Different approaches have been used to detect these cells, including in vitro culture, magnetic bead isolation, and flow cytometry. We review flow cytometric methods for the detection and enumeration of these cells and provide technical suggestions to promote the accurate enumeration of circulating endothelial cells and endothelial progenitor cells. Published 2005 Wiley‐Liss, Inc.