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Tetraspan and beta‐1 integrins expression pattern of the epithelial lung adenocarcinoma cell line A549 and its sensitivity to divalent cations
Author(s) -
Massin Frédéric,
Rubinstein Eric,
Faure Gilbert C.,
Martinet Yves,
Boucheix Claude,
Béné Marie C.
Publication year - 2004
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.20010
Subject(s) - divalent , a549 cell , chemistry , cell culture , integrin , line (geometry) , sensitivity (control systems) , human lung , microbiology and biotechnology , adenocarcinoma , cancer research , lung , cell , medicine , biology , biochemistry , cancer , engineering , genetics , mathematics , organic chemistry , geometry , electronic engineering
Abstract Background Tetraspans are ubiquitous integral transmembrane molecules associated on the cell surface with such adhesion molecules as integrins. Their expression has been shown to vary in tumors, but has seldom been described on lung tumoral epithelial cells, and the conditions required for a proper association of tetraspans and integrins have not yet been fully explored. Methods We investigated the expression of 10 tetraspans and six β1 integrins on the tumoral lung epithelial cell line A549. Cells were examined both in quantitative flow cytometry and as monolayers, under normal or chelated conditions, in order to determine the cation dependency of their expression. Results Five tetraspans and four β1 integrins are expressed on the membrane of A549 cells. Both quantitative and qualitative surface and cytoplasmic modifications of this pattern were induced in chelating conditions, suggesting the importance of divalent cations for the expression of these molecules. Conclusions These data indicate that a specific pattern of tetraspans and integrins, relying strongly on the availability of divalent cations in the microenvironment, is expressed by tumoral epithelial cells. © 2004 Wiley‐Liss, Inc.