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Immunophenotyping is an independent factor for risk stratification in AML
Author(s) -
Repp R.,
Schaekel U.,
Helm G.,
Thiede C.,
Soucek S.,
Pascheberg U.,
Wandt H.,
Aulitzky W.,
Bodenstein H.,
Sonnen R.,
Link H.,
Ehninger G.,
Gramatzki M.
Publication year - 2003
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.10030
Subject(s) - risk stratification , immunophenotyping , stratification (seeds) , factor (programming language) , medicine , risk factor , oncology , computer science , immunology , biology , flow cytometry , programming language , seed dormancy , botany , germination , dormancy
Background Chromosomal abnormalities are one of the most important prognostic factors in acute myeloid leukemia (AML). However, only a limited number of patients have such informative chromosomal abnormalities. The prognostic value of immunophenotyping in this disease is still unclear. Methods Seven hundred and eighty‐three newly diagnosed AML patients treated in the German SHG‐AML trials in 1991 and 1996 were analyzed with a panel of 33 antibodies. Expression was correlated to overall survival, complete remission‐rate, and complete remission duration, and tested in a multivariate analysis including other clinical and biological markers. Results With a median follow‐up of 4.3 years, patients with AML blasts negative for CD9, CD11b, CD13, CD34, and CD41, or positive for CD15, CD33, CD38, CD64, and MPO had superior overall survival. This effect was associated with a significantly higher complete remission rate (CD13, CD34, CD41, and CD64) or a longer complete remission duration (CD9, CD11b, and CD64). Cox‐regression analysis, including cytogenetic, morphologic, and biologic parameters showed CD9, CD13, CD34, and CD64 as independent factors for overall survival. These markers were used for a prognostic score. Patients were pooled in three groups with highly significant differences of overall survival. The prognostic relevance of this score was confirmed in patients with normal karyotype and/or in younger patients ≤ 60 years. Conclusions Immunophenotyping is not only helpful for diagnosis but is of independent significance for prognosis, and may be useful for risk stratification in AML patients. Cytometry Part B (Clin. Cytometry) 53B:11–19, 2003. © 2003 Wiley‐Liss, Inc.