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Comprehensive phenotyping of endothelial cells using flow cytometry 1: Murine
Author(s) -
Grant Dillon,
Wanner Nicholas,
Frimel Matthew,
Erzurum Serpil,
Asosingh Kewal
Publication year - 2021
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.24292
Subject(s) - immunophenotyping , flow cytometry , biology , endothelial stem cell , cell type , endothelium , microbiology and biotechnology , immunology , cluster of differentiation , lymph , pathology , cell , medicine , in vitro , genetics
Abstract The endothelium forms a selective barrier between circulating blood or lymph and surrounding tissue. Endothelial cells play an essential role in vessel homeostasis, and identification of these cells is critical in vascular biology research. However, characteristics of endothelial cells differ depending on the location and type of blood or lymph vessel. Endothelial cell subsets are numerous and often identified using different flow cytometric markers, making immunophenotyping these cells complex. In part 1 of this two part review series, we present a comprehensive overview of markers for the flow cytometric identification and phenotyping of murine endothelial subsets. These subsets can be distinguished using a panel of cell surface and intracellular markers shared by all endothelial cells in combination with additional markers of specialized endothelial cell types. This review can be used to determine the best markers for identifying and phenotyping desired murine endothelial cell subsets.