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OMIP‐066: Identification of Novel Subpopulations of Human Group 2 Innate Lymphoid Cells in Peripheral Blood
Author(s) -
Ohne Yoichiro
Publication year - 2020
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.24046
Subject(s) - innate lymphoid cell , immunophenotyping , flow cytometry , cytometry , peripheral blood , immunology , phycoerythrin , biology , identification (biology) , antigen , acquired immune system , botany
Purpose and Appropriate Sample Types This 14‐color flow cytometry panel was designed to identify newly described subpopulations within human group 2 innate lymphoid cells (ILC2s) and other ILC subsets. This panel also allowed to identify recently reported subpopulations of peripheral blood CRTH2 − c‐Kit + ILCs. We validated this panel mostly in human peripheral blood but also confirmed that the same panel and gating strategy works well in human tonsillar cells. The panel contains a few markers indicating the activation status of ILCs. In addition, phycoerythrin (PE) channel is available for the markers of interest in each study. In the validation studies described here, PE channel was used to test the expression of some markers. These features make this panel applicable for immunophenotyping of ILCs in various disease states. © 2020 International Society for Advancement of Cytometry