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An Evaluation of T‐Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation
Author(s) -
Andrä Immanuel,
Ulrich Hanna,
Dürr Susi,
Soll Dominik,
Henkel Lynette,
Angerpointner Corinne,
Ritter Julia,
Przibilla Sabine,
Stadler Herbert,
Effenberger Manuel,
Busch Dirk H.,
Schiemann Matthias
Publication year - 2020
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.23964
Subject(s) - flow cytometry , cell sorting , cytometry , microbiology and biotechnology , sorting , mapk/erk pathway , cell , p38 mitogen activated protein kinases , primary cell , biology , chemistry , signal transduction , computer science , biochemistry , programming language
Abstract Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter‐induced physical forces. In summary, flow cytometry cell‐sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.