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OMIP‐062: A 14‐Color, 16‐Antibody Panel for Immunophenotyping Human Innate Lymphoid, Myeloid and T Cells in Small Volumes of Whole Blood and Pediatric Airway Samples
Author(s) -
Swieboda Dawid,
Guo Yanping,
Sagawe Sophie,
Thwaites Ryan S.,
Nadel Simon,
Openshaw Peter J.M.,
Culley Fiona J.
Publication year - 2019
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.23907
Subject(s) - immunology , immune system , innate lymphoid cell , immunophenotyping , myeloid , antibody , acquired immune system , innate immune system , cd8 , biology , medicine , antigen
This 14-color, 16-antibody OMIP was designed for enumeration of leukocyte responses in pediatric samples, where sample volumes and cell numbers can be very low. Leukocytes identified by this panel include all major members of the innate lymphoid cell (ILC) family (ILC1s, ILC2s, and ILC3s), natural killer cells (NK cells), granulocytes (neutrophils and eosinophils), T-cells (CD4 and CD8), mucosalassociated invariant T cells (MAIT cells) and NKT-like cells. The protocol was optimized using small volumes of peripheral blood and validated in airway samples obtained from children (< 2 years of age) admitted to a pediatric intensive care unit (PICU). Given this backdrop, this OMIP is widely applicable to clinical research using low volume or paucicellular samples, such as studies of innate and adaptive immune responses in infants and children, with potential clinical application in diagnostics and monitoring of patients by pediatricians.