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How Clinical Flow Cytometry Rebooted Sepsis Immunology
Author(s) -
Monneret Guillaume,
Gossez Morgane,
Aghaeepour Nima,
Gaudilliere Brice,
Venet Fabienne
Publication year - 2019
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.23749
Subject(s) - flow cytometry , sepsis , cytometry , mass cytometry , immunology , immune system , immunosuppression , medicine , biology , phenotype , biochemistry , gene
On May 2017, the World Health Organization (WHO) recognized sepsis as a global health priority by adopting a resolution to improve the prevention, diagnosis, and management of this deadly disease. While it has long been known that sepsis deeply perturbs immune homeostasis by inducing a tremendous systemic inflammatory response, pivotal observations based on clinical flow cytometry indicate that sepsis indeed initiates a more complex immune response that varies over time, with the concomitant occurrence of both pro‐ and anti‐inflammatory mechanisms. As a resultant, some septic patients enter a stage of protracted immunosuppression. This paved the way for immunostimulation approaches in sepsis. Clinical flow cytometry permitted this evolution by drawing a new picture of pathophysiology and reshaping immune trajectories in patients. Additional information from cytometry by time of flight mass cytometry and other high‐dimensional flow cytometry platform should rapidly enrich our understanding of this complex disease. This review reports on landmarks of clinical flow cytometry in sepsis and how this single‐cell analysis technique permitted to breach the wall of decades of unfruitful anti‐inflammatory‐based clinical trials in sepsis. © 2019 International Society for Advancement of Cytometry