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Unstimulated Adult Human B Cells Include an IL‐10+ Population with Suppressive Properties and an Activated Phenotype
Author(s) -
Šunina Marina,
Kaleviste Epp,
Uibo Raivo,
Kisand Kai
Publication year - 2018
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.23636
Subject(s) - regulatory b cells , secretion , immunology , immune system , biology , flow cytometry , population , microbiology and biotechnology , b cell , ex vivo , phenotype , autoimmunity , t cell , interleukin 10 , in vitro , medicine , antibody , endocrinology , genetics , environmental health , gene
B cells with regulatory properties have been recently identified and described in different immune disorders, including autoimmunity, infection, cancer, and allergy. in vitro studies of regulatory B cells are usually performed following prolonged cell culture and stimulation in order to obtain B cells capable of IL‐10 secretion. We describe the isolation of viable IL‐10‐positive B cells directly from ex vivo unstimulated samples using the IL‐10 secretion assay from Miltenyi Biotec, which was originally designed for IL‐10‐positive T cell analysis and isolation. IL‐10‐positive B cells from unstimulated samples represented approximately 2% of all B cells in healthy individuals, suppressed T cell proliferation and were enriched for surface markers of B cell activation. This tool has a potential to boost functional studies of IL‐10‐secreting B cells in health and disease. © 2018 International Society for Advancement of Cytometry