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Morpho‐Functional Characteristics of Bone Marrow Multipotent Mesenchymal Stromal Cells after Activation or Inhibition of Epidermal Growth Factor and Toll‐Like Receptors or Treatment with DNA Intercalator Cisplatin
Author(s) -
Golovynska Iuliia,
Kalmukova Olesia,
Svitina Hanna M.,
Kyryk Vitaliy M.,
Shablii Volodimir A.,
Senchylo Nataliya V.,
Ostrovska Galyna V.,
Dzerzhinskyi Mykola,
Stepanov Yurii V.,
Golovynskyi Sergii,
Ohulchanskyy Tymish Y.,
Liu Liwei,
Garmanchuk Liudmila V.,
Qu Junle
Publication year - 2019
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.23593
Subject(s) - mesenchymal stem cell , microbiology and biotechnology , population , stromal cell , epidermal growth factor , biology , cancer research , bone marrow , multipotent stem cell , chemistry , rankl , receptor , immunology , stem cell , progenitor cell , biochemistry , medicine , activator (genetics) , environmental health
This study is aimed to reveal morphological and functional changes in multipotent mesenchymal stromal cells (MSCs) isolated from the rat bone marrow after: (i) activation of Toll‐like receptors (TLRs) with teichoic acid (TA), (ii) impact on epidermal growth factor (EGF) receptors with activator EGF or inhibitor Herceptin, and (iii) treatment with DNA intercalator Cisplatin. According to our results, TA and EGF cause an increase in the synthesis of glycosaminoglycans, c‐Myc content, and protein in the MSC cytoplasm. It was observed that the cell population in G0 phase decreased and the cell population in G1 phase increased, when compared with control. At the same time, the cell population with a higher nuclear–cytoplasmic ratio (NCR) in S and G2 phases also increased. This indicates the manifestation of the MSC mesenchymal phenotype, exhibiting indirect metabolic signs of the regenerative potential increase. In other experiments, Herceptin was shown to suppress only the stemness signs of MSCs, while Cisplatin seriously affected cell viability in general, reducing synthetic and proliferative activities and causing cell morphology disturbances. © 2018 International Society for Advancement of Cytometry