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OMIP‐048 MC: Quantification of calcium sensors and channels expression in lymphocyte subsets by mass cytometry
Author(s) -
JaraczRos Agnieszka,
Hémon Patrice,
Krzysiek Roman,
Bachelerie Françoise,
SchlechtLouf Géraldine,
GaryGouy Hélène
Publication year - 2018
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.23504
Subject(s) - mass cytometry , flow cytometry , cytometry , lymphocyte subsets , lymphocyte , calcium , chemistry , microbiology and biotechnology , biology , immunology , immune system , biochemistry , t cell , organic chemistry , gene , phenotype
Calcium (Ca 2+ ) signaling controls T‐cell activation and functions. Ca 2+ concentrations are locally detected and controlled by Ca 2+ ‐sensors (STIM1 and 2 detecting the depletion from ER stores channels) and Ca 2+ ‐channels (ORAI1‐3 in the cell membrane and VDAC1 in the outer mitochondrial membrane). We first validated and titrated antibodies to assess the expression of these Ca 2+ ‐sensors and ‐channels in human and murine cells, and further devised a 18‐antibodies mass cytometry panel to characterize their expression in primary murine lymphocyte subsets.