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Tellurium‐based mass cytometry barcode for live and fixed cells
Author(s) -
Willis Lisa M.,
Park Hanuel,
Watson Michael W. L.,
Majonis Daniel,
Watson Jessica L.,
Nitz Mark
Publication year - 2018
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.23495
Subject(s) - mass cytometry , barcode , microscale chemistry , flow cytometry , cytometry , biology , sample preparation , chemistry , computational biology , microbiology and biotechnology , chromatography , computer science , biochemistry , phenotype , mathematics , mathematics education , gene , operating system
Mass cytometry is a revolutionary technology that allows for the simultaneous quantification of >40 different biomarkers with cellular resolution. The biomarkers are detected using metal‐labeled antibodies as well as small‐molecule probes of cell size, viability, and biochemical status. Barcoding is an important component of sample preparation because it reduces processing time, eliminates sample‐to‐sample variation, discriminates cell doublets, reduces the amount of antibody needed, and conserves sample. We developed a thiol‐reactive tellurium‐based barcode, TeMal. TeMal is nontoxic at working concentrations, compatible with metal‐labeled antibodies, and can readily be applied to live or fixed cells, making it advantageous and complementary compared to existing barcoding reagents. We have demonstrated the utility of TeMal by barcoding microscale samples in situ to facilitate analysis of cells from an automated cell culture system using mass cytometry.