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High‐throughput analysis of TUNEL ‐stained sperm using image cytometry
Author(s) -
Arifulin E. A.,
Bragina E. E.,
Kurilo L. F.,
Sheval E. V.
Publication year - 2017
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.23164
Subject(s) - cytometry , sperm , flow cytometry , throughput , tunel assay , andrology , computer science , biology , medicine , pathology , microbiology and biotechnology , immunohistochemistry , telecommunications , wireless
Sperm DNA integrity is an essential factor for successful fertilization and proper pregnancy progression. The terminal deoxynucleotidyl transferase dUTP nick end‐labeling (TUNEL) assay is commonly used for the estimation of the DNA fragmentation index. Analysis of TUNEL‐stained sperm is often performed by flow cytometry, an approach that allows high‐throughput analysis but in which any morphological information is lost. In this study, results of an automated image cytometry estimation of TUNEL‐stained sperms were presented. The results of visual counting and automatic analysis were closely correlated, indicating that image cytometry is suitable for such analysis and may be applied in a clinical setting. © 2017 International Society for Advancement of Cytometry