Premium
OMIP‐035: Functional analysis of natural killer cell subsets in macaques
Author(s) -
Weisgrau Kim L.,
Ries Moritz,
Pomplun Nicholas,
Evans David T.,
Rakasz Eva G.
Publication year - 2016
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.22932
Subject(s) - cd16 , peripheral blood mononuclear cell , immunology , biology , flow cytometry , natural killer cell , cytotoxic t cell , cell , receptor , antigen , in vitro , cd8 , genetics , cd3
This panel was developed to measure the functional capability of natural killer (NK) cell subsets in rhesus macaques ( Macaca mulatta ). It includes markers to determine the frequency of cytokine secreting and cytotoxic NK cell subpopulations in peripheral blood mononuclear cell (PBMC) samples stimulated in vitro with human 721.221 cells. NK cell subsets were defined by the expression of killer cell immunoglobulin‐like receptors (KIRs) Mamu‐KIR3DL01 and Mamu‐KIR3DL05, and differentiation antigens CD16 and CD56. The panel can be used to assess the functional capability of NK cells in a range of normal and pathologic conditions of captive bred rhesus macaques of Indian origin. © 2016 International Society for Advancement of Cytometry