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Platinum‐conjugated antibodies for application in mass cytometry
Author(s) -
Mei Henrik E.,
Leipold Michael D.,
Maecker Holden T.
Publication year - 2016
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.22778
Subject(s) - mass cytometry , flow cytometry , cytometry , analyte , chemistry , antibody , biophysics , chromatography , microbiology and biotechnology , biology , biochemistry , immunology , gene , phenotype
Mass cytometry has overcome limitations of fluorescent single cell cytometry by allowing for the measurement of up to currently ∼40 different parameters on a single cell level. However, the cellular proteome comprises many more potential analytes, and current mass cytometry instrumentation allows for theoretically up to 121 different mass detection channels. The labeling of specific probes with appropriate metal ions is a significant hurdle for exploiting more of mass cytometry's analytical capacity. To this end, we here describe the labeling of antibody with natural abundance or isotopically purified platinum as formulated in cisplatin and circumventing the use of chelator‐loaded polymers. We confirm the utility of cisplatin–antibody‐conjugates for surface, intracellular, and phosphoepitope‐specific immunophenotyping, as well as for application in cell surface CD45‐based barcoding. Cisplatin‐labeling of antibody increases the analytical capacity of the CyTOF ® platform by two channels based on available reagents, and has the potential to add a total of six channels for detection of specific probes, thus helping to better extend the analytical mass range of mass cytometers. © 2015 International Society for Advancement of Cytometry