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Design and validation of conditional ligands for HLA‐B *08:01, HLA‐B *15:01, HLA‐B *35:01, and HLA‐B *44:05
Author(s) -
Frøsig Thomas Mørch,
Yap Jiawei,
Seremet Tina,
Lyngaa Rikke,
Svane Inge Marie,
Thor Straten Per,
Heemskerk Mirjam H. M.,
Grotenbreg Gijsbert M.,
Hadrup Sine Reker
Publication year - 2015
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.22689
Subject(s) - human leukocyte antigen , hla b , major histocompatibility complex , context (archaeology) , flow cytometry , biology , hla a , computational biology , b cell , cytotoxic t cell , immunology , genetics , antigen , in vitro , antibody , paleontology
We designed conditional ligands restricted to HLA‐B*08:01, −B*35:01, and −B*44:05 and proved the use of a conditional ligand previously designed for HLA‐B*15:02 together with HLA‐B*15:01. Furthermore, we compared the detection capabilities of specific HLA‐B*15:01‐restricted T cells using the HLA‐B*15:01 and HLA‐B*15:02 major histocompatibility complex (MHC) multimers and found remarkable differences in the staining patterns detected by flow cytometry. These new conditional ligands greatly add to the application of MHC‐based technologies in the analyses of T‐cell recognition as they represent frequently expressed HLA‐B molecules. This expansion of conditional ligands is important to allow T‐cell detection over a wide range of HLA restrictions, and provide comprehensive understanding of the T‐cell recognition in a given context. © 2015 International Society for Advancement of Cytometry