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A simple flow cytometry‐based barcode for routine authentication of multiple myeloma and mantle cell lymphoma cell lines
Author(s) -
Maïga Sophie,
Brosseau Carole,
Descamps Géraldine,
Dousset Christelle,
GomezBougie Patricia,
Chiron David,
Ménoret Emmanuelle,
Kervoelen Charlotte,
Vié Henri,
Cesbron Anne,
MoreauAubry Agnès,
Amiot Martine,
PellatDeceunynck Catherine
Publication year - 2015
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.22643
Subject(s) - cell culture , flow cytometry , human leukocyte antigen , cytometry , computational biology , biology , computer science , immunology , antigen , genetics
lines are widely used in laboratories for in vitro experi-ments, especially for investigating abnormal hallmarks in can-cer cells and identifying therapeutic targets. Human cell linesare typically derived in academic laboratories from a widerange of cancer samples. To achieve a representation of intra-cancer heterogeneity, several laboratories, including ours, haveestablished cell line collections. However, the establishmentand maintenance of such collections significantly increase therisk of cross-contaminations and misidentification of celllines, leading to the publication of false data/interpretation(1). In addition to the risk of cross-contamination, widelyused cell lines can be described in contrasting manners for aparticular feature (e.g., the JJN3 myeloma cell line appearseither TP53