Image‐based cell‐resolved screening assays in flow

A parallel microfluidic cytometer (PMC) is based on a one‐dimensional (1D) scanning detector, a parallel array of flow channels, and new multiparameter analysis algorithms that operate on low‐pixel‐count 1D images. In this article, we explore a series of image‐based live‐ and fixed‐cell screening assays, including two NF‐kB nuclear translocations and T‐cell capping. We then develop a new multiparametric linear weighted classifier that achieves a Z ′ factor sufficient for scaled pharmaceutical discovery with Jurkat cells in suspension. We conclude that the PMC should have the throughput and statistical power to permit a new capability for image‐based high‐sample‐number pharmaceutical screening with suspension samples. © 2014 International Society for Advancement of Cytometry