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Flow cytometry applications for the analysis of chemokine receptor expression and function
Author(s) -
Anselmo Achille,
Mazzon Cristina,
Borroni Elena Monica,
Bonecchi Raffaella,
Graham Gerard J.,
Locati Massimo
Publication year - 2014
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.22439
Subject(s) - chemokine receptor , chemokine , flow cytometry , microbiology and biotechnology , receptor , ccr1 , biology , function (biology) , cc chemokine receptors , intracellular , computational biology , immunology , biochemistry
Chemokine receptors play an important role in leukocyte migration, both in physiological and pathological conditions, and the interest in new methodologies for their detection is increasing. In this review, we focused on chemokine receptors detection through flow cytometric approaches, including the use of specific antibodies and fluorescent chemokines, and on approaches aimed at the analysis of their functions, from intracellular trafficking to signaling activities. © 2014 International Society for Advancement of Cytometry