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New violet‐excitable reagents for multicolor flow applications
Author(s) -
Abrams Barny,
Diwu Zhenjun,
Guryev Oleg,
Suni Maria,
Dubrovsky Tim
Publication year - 2013
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.22309
Subject(s) - reagent , flow cytometry , fluorescence , conjugate , chemistry , chromatography , physics , biology , optics , microbiology and biotechnology , mathematics , mathematical analysis
We have recently added three new fluorophores–BD Horizon™ V450, BD Horizon V500, and BD Horizon V550 (V450, V500, and V550; BD Biosciences, San Jose, CA) to our existing AmCyan product, forming a group of four violet‐excitable dyes from which we have produced functional antibody conjugates. These conjugates, with emission maxima that range from 450 to 535 nm, are compatible with multilaser flow cytometry (FCM) and can be used for polychromatic FCM in three‐color or two‐color combinations; in fact, V500 fills a spectral opening that has thus far not been exploited by other manufacturers of FCM reagents. We here report that conjugates based on BD Horizon dyes performed well within a useful sensitivity range, established by testing a representative group of violet‐excitable FCM reagents currently available, and that V500 has better compatibility with FITC in multicolor applications than does AmCyan. © 2013 International Society for Advancement of Cytometry