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OMIP‐006: Phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry
Author(s) -
Murdoch David M.,
Staats Janet S.,
Weinhold Kent J.
Publication year - 2012
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.22024
Subject(s) - flow cytometry , phenotype , cytometry , biology , microbiology and biotechnology , computational biology , genetics , gene
This panel was optimized for the enumeration and phenotypic characterization of T regulatory cells (Tregs) within the CD4 + T‐cell pool using human peripheral blood mononuclear cells (PBMC) using intranuclear and intracellular staining methods. The panel was optimized for HIV + clinical trial specimens through the use of HIV‐infected and normal donor PBMC. Because the panel is to be used in the context of testing cryopreserved PBMC obtained from multiple sites participating in clinical trials, it was essential to develop an assay that performed well using cryopreserved PBMC. Other tissue types have not been tested. © 2012 International Society for Advancement of Cytometry.