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Flow cytometry as a tool for analyzing changes in Plasmodium falciparum cell cycle following treatment with indol compounds
Author(s) -
Schuck Desirée Cigaran,
Ribeiro Ramira Yuri,
Nery Arthur A.,
Ulrich Henning,
Garcia Célia R. S.
Publication year - 2011
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.21136
Subject(s) - flow cytometry , plasmodium falciparum , cytometry , biology , serotonin , cell cycle , melatonin , tryptamine , red blood cell , staining , cell , biochemistry , microbiology and biotechnology , immunology , malaria , endocrinology , receptor , genetics
Melatonin and its derivatives modulate the Plasmodium falciparum and Plasmodium chabaudi cell cycle. Flow cytometry was employed together with the nucleic acid dye YOYO‐1 allowing precise discrimination between mono‐ and multinucleated forms of P. falciparum ‐infected red blood cell. The use of YOYO‐1 permitted excellent discrimination between uninfected and infected red blood cells as well as between early and late parasite stages. Fluorescence intensities of schizont‐stage parasites were about 10‐fold greater than those of ring‐trophozoite form parasites. Melatonin and related indolic compounds including serotonin, N ‐acetyl‐serotonin and tryptamine induced an increase in the percentage of multinucleated forms compared to non‐treated control cultures. YOYO‐1 staining of infected erythrocyte and subsequent flow cytometry analysis provides a powerful tool in malaria research for screening of bioactive compounds. © 2011 International Society for Advancement of Cytometry

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