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Comparison of parameter‐adapted segmentation methods for fluorescence micrographs
Author(s) -
Held Christian,
Palmisano Ralf,
Häberle Lothar,
Hensel Michael,
Wittenberg Thomas
Publication year - 2011
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.21122
Subject(s) - segmentation , artificial intelligence , computer science , computer vision , image segmentation , pattern recognition (psychology) , reproducibility , scale space segmentation , usable , mathematics , statistics , world wide web
Abstract Interpreting images from fluorescence microscopy is often a time‐consuming task with poor reproducibility. Various image processing routines that can help investigators evaluate the images are therefore useful. The critical aspect for a reliable automatic image analysis system is a robust segmentation algorithm that can perform accurate segmentation for different cell types. In this study, several image segmentation methods were therefore compared and evaluated in order to identify the most appropriate segmentation schemes that are usable with little new parameterization and robustly with different types of fluorescence‐stained cells for various biological and biomedical tasks. The study investigated, compared, and enhanced four different methods for segmentation of cultured epithelial cells. The maximum‐intensity linking (MIL) method, an improved MIL, a watershed method, and an improved watershed method based on morphological reconstruction were used. Three manually annotated datasets consisting of 261, 817, and 1,333 HeLa or L929 cells were used to compare the different algorithms. The comparisons and evaluations showed that the segmentation performance of methods based on the watershed transform was significantly superior to the performance of the MIL method. The results also indicate that using morphological opening by reconstruction can improve the segmentation of cells stained with a marker that exhibits the dotted surface of cells. © 2011 International Society for Advancement of Cytometry