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Just compensation?
Author(s) -
Rajwa Bartek
Publication year - 2011
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.21118
Subject(s) - detector , cytometry , signal (programming language) , nonlinear system , compensation (psychology) , computer science , flow cytometry , mathematics , physics , medicine , telecommunications , psychology , quantum mechanics , psychoanalysis , immunology , programming language
IN their recent article entitled ‘‘Improved compensation in flow cytometry by multivariable optimization,’’ Sug ar et al. (1) made the claim that introduction of double-stained controls improves the quality of the compensation process in flow cytometry. However, it seems that the conclusions presented by the authors may be well based on a logical fallacy. As demonstrated by Bagwell and Adams (2) and Roederer (3), cytometry compensation is in fact a special case of linear spectral unmixing. As such, the phenomenon known in cytometry as ‘‘spectral spillover’’ may be modeled using a basic linear spectral mixture equation. For every single measured cell