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In vivo ultra‐fast photoacoustic flow cytometry of circulating human melanoma cells using near‐infrared high‐pulse rate lasers
Author(s) -
Nedosekin Dmitry A.,
Sarimollaoglu Mustafa,
Ye JianHui,
Galanzha Ekaterina I.,
Zharov Vladimir P.
Publication year - 2011
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.21102
Subject(s) - in vivo , flow cytometry , melanoma , circulating tumor cell , cancer research , photoacoustic imaging in biomedicine , metastasis , materials science , cytometry , biomedical engineering , medicine , cancer , biology , immunology , optics , physics , microbiology and biotechnology
The circulating tumor cells (CTCs) appear to be a marker of metastasis development, especially, for highly aggressive and epidemically growing melanoma malignancy that is often metastatic at early stages. Recently, we introduced in vivo photoacoustic (PA) flow cytometry (PAFC) for label‐free detection of mouse B16F10 CTCs in melanoma‐bearing mice using melanin as an intrinsic marker. Here, we significantly improve thespeed of PAFC by using a high‐pulse repetition rate laser operating at 820 and 1064 nm wavelengths. This platform was used in preclinical studies for label‐free PA detection of low‐pigmented human CTCs. Demonstrated label‐free PAFC detection, low level of background signals, and favorable safety standards for near‐infrared irradiation suggest that a fiber laser operating at 1064 nm at pulse repetition rates up to 0.5 MHz could be a promising source for portable clinical PAFC devices. The possible applications can include early diagnosis of melanoma at the parallel progression of primary tumor and CTCs, detection of cancer recurrence, residual disease and real‐time monitoring of therapy efficiency by counting CTCs before, during, and after therapeutic intervention. Herewith, we also address sensitivity of label‐free detection of melanoma CTCs and introduce in vivo CTC targeting by magnetic nanoparticles conjugated with specific antibody and magnetic cells enrichment. © 2011 International Society for Advancement of Cytometry