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Measurement and autocorrelation analysis of two‐dimensional light‐scattering patterns from living cells for label‐free classification
Author(s) -
Pan YongLe,
Berg Matthew J.,
Zhang Samuel ShaoMin,
Noh Heeso,
Cao Hui,
Chang Richard K.,
Videen Gorden
Publication year - 2011
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.21036
Subject(s) - autocorrelation , scattering , light scattering , fluorescence , optics , maxima , biological system , physics , computer science , statistics , biology , mathematics , art , performance art , art history
We incorporate optics and an ICCD to record the two‐dimensional angular optical scattering (TAOS) patterns retrieved from single aerosolized cells. We analyze these patterns by performing autocorrelations and demonstrate that we are able to retrieve cell size from the locations of the secondary maxima. Additional morphological information is contained in the autocorrelation functions and decay rate of the heights of the autocorrelation peaks. We demonstrate these techniques with C6 and Y79 cells, which are readily distinguishable. One key advantage of this methodology is that there is no requirement for antibody and fluorescent labeling molecules. Published 2011 Wiley‐Liss, Inc.